Latest Histology research

Top 10 this week

Week of 1 November 2026

1. 1

   Systemic and Histopathological Effects of Bromelain-Based Enzymatic Debridement in Human Burns-A Systematic Review

   Medical sciences (Basel, Switzerland) · Q1 journal · SR/MA of RCTs · Evidence level 1 (High) · 13 October 2026

   Across 12 human studies, none measured systemic effects and tissue changes in the same burn patients: ten studies included 970 patients, while two tissue studies included just nine. C-reactive protein and procalcitonin were higher after enzymatic than surgical debridement during the first week, but biopsies were described only qualitatively. The mechanisms behind this systemic response remain untested in paired tissue and blood data.

2. 2

   Citrulline Supplementation Improves Aortic Vasodilation and Prevents Aortic Thickening Only in Female Rats

   Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists · Q1 journal · RCT · Evidence level 1 (High) · 30 October 2026

   In this 12-week study of adult rats, citrulline improved aortic relaxation in females, while treated males showed no benefit. Female rats also had less elastic-fiber disruption and a thinner inner aortic layer, but these animal findings do not establish benefit in people; whether sex or hormonal status shapes any human response remains unknown.

3. 3

   Computational image features of immune architecture reveal molecular patterns associated with outcome and risk of recurrence in microsatellite-stable or MSI-low colon cancers

   European journal of cancer (Oxford, England : 1990) · Q1 journal · Prospective cohort · Evidence level 2 (Moderate) · 29 October 2026

   In 461 patients with microsatellite-stable or MSI-low colon cancer, a tool measuring immune-cell arrangement on routine slides identified high- and low-risk groups, with about three times the progression hazard in the high-risk group within both stage II and stage III disease. Adding the score improved prediction beyond clinical factors, but prospective validation is still needed.

4. 4

   MGP_HNSC32: A H&E Morphology-Derived 32-Gene Prognostic Signature in Head and Neck Squamous Cell Carcinoma

   Cancer medicine · Q1 journal · Prospective cohort · Evidence level 2 (Moderate) · 1 November 2026

   A 32-gene score inferred from routine H&E slides separated high- and low-risk patients in an independent head and neck cancer cohort, with the high-risk group showing a 40% higher progression hazard at the prespecified cutoff. The continuous score was not significantly associated with progression, so this proof-of-concept needs further validation before clinical use.

5. 5

   Diabetes-Associated Fibrin Enrichment in Cerebral Thrombi and Its Procedural Implications for Mechanical Thrombectomy

   Journal of clinical medicine · Q1 journal · Cohort · Evidence level 3 (Low) · 20 October 2026

   In a study of 312 thrombectomy patients, diabetes was associated with 6.7 percentage points more fibrin in retrieved clots after adjustment; red blood cell and platelet content did not differ significantly. The extra fibrin did not measurably explain differences in reperfusion or other procedural outcomes, so its clinical significance during thrombectomy remains unclear.

6. 6

   Raman Spectroscopy for Glioma Diagnosis Toward Precision Neuro-Oncology: A Systematic Review

   Critical reviews in oncology/hematology · Q1 journal · SR of cohorts · Evidence level 2 (Moderate) · 31 October 2026

   A review of 47 studies found Raman-based technologies consistently distinguished glioma tissue, assessed tumor margins and supported molecular characterization, including during surgery. But varied methods, mostly retrospective single-center research and limited external validation leave their performance in broader clinical practice uncertain; prospective multicenter studies are needed before routine use.

7. 7

   AGE-RELATED BIOMECHANICAL AND STRUCTURAL REMODELING OF HUMAN MIDDLE CEREBRAL ARTERIES

   Acta biomaterialia · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 30 October 2026

   Human middle cerebral arteries from older people were stiffer and showed greater stress relaxation, alongside higher collagen and glycosaminoglycan content and denser collagen in the vessel wall. The tissue study links age-related structural changes to altered artery mechanics, but it does not establish how these changes affect an individual's stroke risk.

8. 8

   The Mouse Retina as an Evolving Model System: From Layered Anatomy to Cell-Type-Resolved Regenerative Repair

   Experimental eye research · Q1 journal · Review / opinion · Evidence level 5 (Expert Opinion) · 27 October 2026

   This review traces how genetics, cell-level analysis and advanced imaging have expanded what mouse retina studies can measure. It stresses that cell survival, visual function, nerve regrowth, circuit reconnection and behavior are separate outcomes, so combining readouts and testing findings in human-relevant tissue and larger-eye models is essential before drawing clinical conclusions.

9. 9

   Glucose transport as a design constraint in white-zone meniscus tissue engineering: a reaction-diffusion framework

   Frontiers in bioengineering and biotechnology · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 15 October 2026

   In a one-dimensional model of a tear 2.5 mm into the meniscus, native glucose transport stayed below 0.30 of the reference concentration, while a hypothetical sustained source reached 0.40 within 4 hours. These normalized levels are not validated healing targets, and the model does not show that tissue heals; it points to transport experiments for future testing.

10. 10

    A methodological evaluation of the immunohistochemistry techniques for peri-implant tissues. A systematic review

    Journal of histotechnology · Q2 journal · SR of cohorts · Evidence level 2 (Moderate) · 27 October 2026

    In this review of human studies, diseased implant tissues showed more inflammation, low oxygen, new blood vessels and marginal bone loss than healthy tissues. Immunohistochemistry can distinguish these tissue states, but differences in antibody choice and laboratory methods make results hard to compare; validated antibodies and standardized protocols are needed before clinical use can be more reliable.

Paper of the day, last two weeks

• Modelling Medial Degenerative Features by Enzymatic Digestion to Evaluate Disease-Relevant Structure-Function Relationships in the Thoracic Aorta

  Acta biomaterialia · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 5 November 2026

  In treated porcine and human aortic tissue, degrading elastin, collagen, or proteoglycans changed stiffness, stretch behavior, and energy loss in distinct ways. The controlled model suggests several routes by which matrix degeneration can make the aortic wall more vulnerable to failure, but whether these mechanical changes predict dissection risk in patients remains unknown.

• Complex 3D cytoarchitecture of the anterior horn of the human sacral spinal cord revealed by light sheet fluorescence microscopy

  Brain structure & function · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 5 November 2026

  Human spinal cord cytoarchitecture revealed with light-sheet microscopy

• Non-invasive assessment of microvascular changes in a Duchenne muscular dystrophy rat model with near-infrared spectroscopy

  Neuromuscular disorders : NMD · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 24 October 2026

  In six Duchenne muscular dystrophy rats and six controls, near-infrared readings showed reduced oxygen extraction in skeletal muscle and strongly tracked muscle microvessel structure. Brain oxygenation did not differ between groups; the small study supports further testing of this non-invasive approach, but validation across disease stages and tissues is still needed.

• Axon Content of the Circumpapillary Nerve Fiber Layer in Experimental Glaucoma

  Investigative ophthalmology & visual science · Q1 journal · Cross-sectional · Evidence level 3 (Low) · 2 November 2026

  In 22 monkeys with experimental glaucoma, a location-adjusted model estimated optic nerve axon counts from OCT scans, with a mean difference of just 25 axons from counts obtained after death. However, individual estimates had very wide limits of agreement, so the model's accuracy for a given eye remains uncertain; validation in people is also needed.

• Hydraulic Permeability of Retinal and Subretinal Layers: Experimental Quantification and Computational Modeling

  Experimental eye research · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 31 October 2026

  In porcine tissue, neuroretina permeability was about 10,000 times lower? Wait values 10^-14 vs 10^-17 = 1,000 times higher than the RPE-BrM-choroid complex. Modeling found that this outer-layer complex dominated fluid transport, while retinal strain had little predicted effect. Formalin fixation preserved measured permeability and improved reproducibility, but how well these ex vivo results translate to human eyes remains to be tested.

• Label- and Slide-Free Multispectral Quantitative Epi-Illumination Deep-Ultraviolet Microscopy

  BME frontiers · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 30 October 2026

  Novel label-free microscopy with quantitative imaging of fresh tissue

• Reduced cortical bone strength and altered osteocyte canaliculi despite preserved bone mass in male Becn 1+/- mice

  Bone · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 25 October 2026

  In 14-week-old male mice with one reduced copy of Becn1, bone mass, structure and mineral density were similar to controls, but bending strength and tissue hardness were lower. Osteocyte channels were also shorter and wider, alongside signs of altered local matrix remodeling. The findings point to bone quality, not mass alone, as a concern in this mouse model; relevance to people remains unknown.

• Reduced cortical bone strength and altered osteocyte canaliculi despite preserved bone mass in male Becn 1+/- mice

  Bone · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 25 October 2026

  In 14-week-old male mice with one reduced copy of Becn1, bone mass, structure and mineral density were similar to controls, but bending strength and tissue hardness were lower. Osteocyte channels were also shorter and wider, alongside signs of altered local matrix remodeling. The findings point to bone quality, not mass alone, as a concern in this mouse model; relevance to people remains unknown.

• Reduced cortical bone strength and altered osteocyte canaliculi despite preserved bone mass in male Becn 1+/- mice

  Bone · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 25 October 2026

  In 14-week-old male mice with one reduced copy of Becn1, bone mass, structure and mineral density were similar to controls, but bending strength and tissue hardness were lower. Osteocyte channels were also shorter and wider, alongside signs of altered local matrix remodeling. The findings point to bone quality, not mass alone, as a concern in this mouse model; relevance to people remains unknown.

• A multiscale confocal-finite element method to relate single-osteocyte Wnt/β-catenin activation to lacunar wall strain in murine cortical bone

  JBMR plus · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 6 October 2026

  Confocal imaging quantifies osteocyte signaling and local matrix strain

• Morphological characteristics of corneal endothelial cells in a healthy southern Han Chinese population across the entire life cycle and evaluation of age-associated endothelial morphological indicators

  Frontiers in medicine · Q1 journal · Cross-sectional · Evidence level 3 (Low) · 8 October 2026

  In specular microscopy of 13,820 healthy southern Han Chinese volunteers aged 3 to 99, endothelial cell density declined by an estimated 0.42% per year of age, while corneal thickness showed no significant age association. Hexagonality tracked age most sensitively, and cell-size variability and largest-cell size were the most consistent indicators of age-related shape changes; these cross-sectional findings do not establish clinical biomarkers.

• Morphology and ultrastructure of pharyngeal sense organs of Drosophila larvae

  eLife · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 23 October 2026

  Detailed sensory-organ architecture and ultrastructure by microscopy

• Histological analysis of the spatio-temporal development of porcine knee cartilage in embryonic and early postnatal stages

  Animal models and experimental medicine · Q1 journal · Unclassified · Evidence level 5 (Expert Opinion) · 22 October 2026

  Maps cartilage development using multiple stains and histological morphology

Evidence summaries in Histology

• Epithelial Tissue